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Volume 07 Issue 4
August 1992
    Citation: PENG Hai, CHEN Xue-Ren, WU Jian-Hua, GONG Jie-Xun. Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA .VIROLOGICA SINICA, 1992, 7(4) : 426.
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    Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA

    • 1.

      Shanghai Institute of Biochemistry

    • 2.

      Academia Sinica

    • 3.

      Shanghai 200031

    • Available online: 05 December 1992
    • In this paper we have designed and constructed an antisense gene which is complementary with the assembly origin of TMV-RNA, then a intermediate vector which contains the expressing unitgconsisting of CaMV 35S pro. moter, antisense RNA gene and poly A signal, as well as the NPT-Ⅱ expressing unit was constructed, The NPT-Ⅱ is essential for the selection and transformation of plants, Thus, the successful construction of this interme diate vector will faciliate obtaining the transgenic plants.
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      Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA

      • 1. Shanghai Institute of Biochemistry
      • 2. Academia Sinica
      • 3. Shanghai 200031

      Abstract: In this paper we have designed and constructed an antisense gene which is complementary with the assembly origin of TMV-RNA, then a intermediate vector which contains the expressing unitgconsisting of CaMV 35S pro. moter, antisense RNA gene and poly A signal, as well as the NPT-Ⅱ expressing unit was constructed, The NPT-Ⅱ is essential for the selection and transformation of plants, Thus, the successful construction of this interme diate vector will faciliate obtaining the transgenic plants.

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