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Volume 08 Issue 1
February 1993
    Citation: CHENG Li, CHEN Man, CHEN Wen-Kai, GUO Shu-Fang. Preparation and Application of Biotin-labelled Human Cytomegalovirus Nucleic Acid Probe .VIROLOGICA SINICA, 1993, 8(1) : 25.
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    Preparation and Application of Biotin-labelled Human Cytomegalovirus Nucleic Acid Probe

    • 1.

      Virus Researcli Institute

    • 2.

      Hubei Medical College

    • Available online: 05 March 1993
    • A diagnostic assay has been developed to detect human cytomegalovirus (HCMV)DNA in clinical specimens with biotin-labelled DNA probe, cloned fragment of HCMV strain ADl69.The biotin-labelled probe can detect 100 pg of homologous DNA and does not hybridize with DNA from other herpesvirus or human embryonic lung cells(HEL) and Hep-2 cells. 30 umbilical cord blood and 10 urine specimens from pregnant women were examined by hybridization assay with biotin-labelled probe. Among 30 umbilical cord blood specimens,...
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      Preparation and Application of Biotin-labelled Human Cytomegalovirus Nucleic Acid Probe

      • 1. Virus Researcli Institute
      • 2. Hubei Medical College

      Abstract: A diagnostic assay has been developed to detect human cytomegalovirus (HCMV)DNA in clinical specimens with biotin-labelled DNA probe, cloned fragment of HCMV strain ADl69.The biotin-labelled probe can detect 100 pg of homologous DNA and does not hybridize with DNA from other herpesvirus or human embryonic lung cells(HEL) and Hep-2 cells. 30 umbilical cord blood and 10 urine specimens from pregnant women were examined by hybridization assay with biotin-labelled probe. Among 30 umbilical cord blood specimens,...

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