Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments
Abstract: Based on the systematic analysis of HFRSV strain 76/118 (Hantaan virus) M and S gene with personal computer, the endonucleased fragments of tha two gene cDNA were subcloned for smaller gene probes and expression studies afterwards. The fragment containing G2 gene and the HindⅢ -BamH Ⅰ 437bp fragment of S gene were cloned into pX62, the positive recombinants of the former were selectecd by inactivation of XylE gene, a chromogenic selective marker, while the latter did not inactivate XylE gene expression, in ...