Citation: JIA Dong-Xiang, WANG Mei-Xian, WANG Jing-Yi, XU Zhi-Kai. Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments .VIROLOGICA SINICA, 1993, 8(1) : 53.

Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments

  • Available online: 05 March 1993
  • Based on the systematic analysis of HFRSV strain 76/118 (Hantaan virus) M and S gene with personal computer, the endonucleased fragments of tha two gene cDNA were subcloned for smaller gene probes and expression studies afterwards. The fragment containing G2 gene and the HindⅢ -BamH Ⅰ 437bp fragment of S gene were cloned into pX62, the positive recombinants of the former were selectecd by inactivation of XylE gene, a chromogenic selective marker, while the latter did not inactivate XylE gene expression, in ...

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    Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments

    • 1. The Fourth Military Medical University
    • 2. Xi an 71OO32Navy General Hospital of PLA
    • 3. Beijing 100037

    Abstract: Based on the systematic analysis of HFRSV strain 76/118 (Hantaan virus) M and S gene with personal computer, the endonucleased fragments of tha two gene cDNA were subcloned for smaller gene probes and expression studies afterwards. The fragment containing G2 gene and the HindⅢ -BamH Ⅰ 437bp fragment of S gene were cloned into pX62, the positive recombinants of the former were selectecd by inactivation of XylE gene, a chromogenic selective marker, while the latter did not inactivate XylE gene expression, in ...

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