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Volume 08 Issue 1
February 1993
    Citation: SHU Zuo-Wei, XUE Qi-Han. Purification and Serology of Sweet Potato Feathery Mottle Virus .VIROLOGICA SINICA, 1993, 8(1) : 84.
    shu

    Purification and Serology of Sweet Potato Feathery Mottle Virus

    • 1.

      Institute of Genetics

    • 2.

      Jiangsu Academy of Agricultural Sciences

    • Available online: 05 March 1993
    • Sweet potato feathery mottle virus (SPEMV) was purified from infected I.Nil leaves using ultracentrifugation and CsCl step gradient centrifugations. Virus yields of 13—15 mg Per kilogram of infected Ieaf tissue were obtained. The normal particle lengths of the SPFMV was 820—860nm. The virus obtained was highly infectious and a good immunogen. Purified virus had a OD260/OD280 ratio of 1.21.
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      Purification and Serology of Sweet Potato Feathery Mottle Virus

      • 1. Institute of Genetics
      • 2. Jiangsu Academy of Agricultural Sciences

      Abstract: Sweet potato feathery mottle virus (SPEMV) was purified from infected I.Nil leaves using ultracentrifugation and CsCl step gradient centrifugations. Virus yields of 13—15 mg Per kilogram of infected Ieaf tissue were obtained. The normal particle lengths of the SPFMV was 820—860nm. The virus obtained was highly infectious and a good immunogen. Purified virus had a OD260/OD280 ratio of 1.21.

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