Comparison of Four Serological Methods for Detecting CMV and TMV in the Crude Extracts of Diseased Tobacco Leaves
Abstract: Three ELISA and an SA-test methods were used to detect CMV and TMV in the crude extracts of diseased plant. For the sensitivity, Dot-ELISA method is the best with maximum dilution point of 1.282.5610~(-3), followed by DSM-ELISA and SPA-ELISA (6.410~(-2)), SA-test is lower(0.8 10~(-2)). For the nonspecific reaction, SPA-ELISA is lower than DSM-ELISA. When the antisera are absorbed by the extracts from the health plants, it was avoided to a great extent in Dot-ELISA and SA-test.