Detection of Epstein-Barr Virus Gene in Biopsied Tissue and Nasopharyngeal Epithelial Cells by Polymerase Chain Reaction
Abstract: The amplifying target sequence for Polymerase Chain Reaction(PCR) was chosen within the Internal Repeat Ⅰ (IR Ⅰ) of EBV genome. We used PCR of the primers to detect 31 biopsied specimens of different disease and 4 nasopharyngeal epithelial cultures. EBV genomes were detected in differentiated nasopharyngeal carcinoma(NPC)biopsies 5/5, poorly differentiated NPC 4/4, Hodgkin s Disease (HD) 3/5, non-Hodgkin s Disease (NHD) 0/2. Other head and neck tumors 1/6, chronic inflammatory nasopharyngeal 0/5, normal nas...