Mutagenesis and Cloning of HIV-1 Protease Gene by Polymerase Chain Reaction

In the present work, the primers HIV-1 Pr1 and HIV-1 Pr2 were synthesized in which the EcoRI and Hind Ⅲ recognition sites as well as the termination codons were designed for convenience of subsequent cloning and expression Micrograms of target gene sequence was produced by 30 rounds of amplification with pJG423 as a template The amplified gene was digested with both EcoRI and Hind Ⅲ and ligated to pUC19 predigested with the same restriction endonucleases. The recombinant plasmid pUPR1 nd pUPR2 were identifi...