Citation: TUN Yun-Chao, CA Yi-Quan. Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe .VIROLOGICA SINICA, 1993, 8(4) : 310-315.

Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe

  • Available online: 05 December 1993
  • A rapid method for detection of HBV-DNA was developed. This assay was based on the hybridization of biotinylated oligonucleotide probe to target DNA immobolized on nitrocellulose membrane which was then incubated with avidin-alkaline phosphatase conjugator and measured with chromogenic substrates. The practicality of the method was investigated. It was found that the method was fast (4hr),sensitive(1-10pg)and expedient for laboratory and clinical detection of HBV-DNA.

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    Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe

    • 1. Wuhan Institute of Virology

    Abstract: A rapid method for detection of HBV-DNA was developed. This assay was based on the hybridization of biotinylated oligonucleotide probe to target DNA immobolized on nitrocellulose membrane which was then incubated with avidin-alkaline phosphatase conjugator and measured with chromogenic substrates. The practicality of the method was investigated. It was found that the method was fast (4hr),sensitive(1-10pg)and expedient for laboratory and clinical detection of HBV-DNA.

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