Detection of Potato Virus X Y and S by Enzyme Linked Immunosorbent Assay on Nitrocellulose Membranes (Dot-ELISA)
Abstract: Purified potato virus Y, X, and S were detected by indirect enzyme-linked immunosorbent assay (ELISA) on nitrocellulose membranes. Potato virus Y, X, and S in infected tobacco, sprouts of potato tubers, rose end of dormant tubers were detected respectively. The results indicated that the small amount of sample required permit the detection of as little as 2.76pg, PVY, 2.835pg PVX and 5.83pg PVS in purified preparations. PVX was detected in infected tobacco、rose end of dormant tubers and sprouts of potato tu...