Detection of I-IPV- 18 DNA with PCR

The DNA fragment(F)on the Upetream Regulatory Region(URR)of HPV-18 DNA was amplified with PCR.Two control experiments here confirmed the PCR specificity.The coneentration ofMg in PCR buffer affected mostly the specificity of PCR,the higher the coneentration of Mg,thelower the specificity,Moreover,nine out of seventeen(53%)DNA samples of cervical carcinoma tissues were detected with PcR for the presence of HVP-18 URR F sequences.It's implied that the re-sult may be an evidence of the relationship between HPV-18 and cervical carcinoma.