Citation: JIANG Gong, KE Li-Hua. Detection of I-IPV- 18 DNA with PCR .VIROLOGICA SINICA, 1994, 9(1) : 7.

Detection of I-IPV- 18 DNA with PCR

  • Available online: 05 March 1994
  • The DNA fragment(F)on the Upetream Regulatory Region(URR)of HPV-18 DNA was amplified with PCR.Two control experiments here confirmed the PCR specificity.The coneentration ofMg in PCR buffer affected mostly the specificity of PCR,the higher the coneentration of Mg,thelower the specificity,Moreover,nine out of seventeen(53%)DNA samples of cervical carcinoma tissues were detected with PcR for the presence of HVP-18 URR F sequences.It's implied that the re-sult may be an evidence of the relationship between HPV-18 and cervical carcinoma.

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    Detection of I-IPV- 18 DNA with PCR

    • 1. Wuhan Institute of Virology,Chinese Academy of Science

    Abstract: The DNA fragment(F)on the Upetream Regulatory Region(URR)of HPV-18 DNA was amplified with PCR.Two control experiments here confirmed the PCR specificity.The coneentration ofMg in PCR buffer affected mostly the specificity of PCR,the higher the coneentration of Mg,thelower the specificity,Moreover,nine out of seventeen(53%)DNA samples of cervical carcinoma tissues were detected with PcR for the presence of HVP-18 URR F sequences.It's implied that the re-sult may be an evidence of the relationship between HPV-18 and cervical carcinoma.

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