Citation: SHAO Yi-Bin, GU Qin, ZENG Rong-Fang. The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration .VIROLOGICA SINICA, 1994, 9(1) : 70.

The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration

  • Available online: 05 March 1994
  • According to the phenomenon that HEPES could enhance CPE in rabies virus infected cell and accelerate the plaques formation,the virulence of rabies virus(RFD strain)was detected with 3 differentmethods includig RHPFT,MC-plaques test and mollse i.c,test.The results showed that the sensibility of 3 detecting methods was similar but RHPFT was more rapid and simple than the latter two,Theresult of RHPFT methed could be obtained earlier about 6-7 days than MC-plaques test which everused as a routine assay in rabies virus titration.RIlPFT could also be used for rabies neutralizing anti-body assay.

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    The Application of Rapid HEPES-plaques-forming Test(RHPFT)to Rabies Virus Titration

    • 1. Institute of Shanghai Biological Products

    Abstract: According to the phenomenon that HEPES could enhance CPE in rabies virus infected cell and accelerate the plaques formation,the virulence of rabies virus(RFD strain)was detected with 3 differentmethods includig RHPFT,MC-plaques test and mollse i.c,test.The results showed that the sensibility of 3 detecting methods was similar but RHPFT was more rapid and simple than the latter two,Theresult of RHPFT methed could be obtained earlier about 6-7 days than MC-plaques test which everused as a routine assay in rabies virus titration.RIlPFT could also be used for rabies neutralizing anti-body assay.

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