For best viewing of the website please use Mozilla Firefox or Google Chrome.
Citation: WANG Jia-Wang, HUANG Yong-Xiu, TAN Ye-Beng, LI Shou-Dong, JI Xi-Feng. PCR Amplification CloningProtein Gene and Location of Major Capsid Of Baculovirus [J].VIROLOGICA SINICA, 1994, 9(2) : 130-137.

PCR Amplification CloningProtein Gene and Location of Major Capsid Of Baculovirus

  • he major eapeid protein gene(vp39)of Autographa californica nuclear polyhedrosis virus(AcNPV)was amplified successfully by PCR technique and cloned into pBluescript SK(+).With pure AcNPVvp39 gene as a probe,the major capeid protein gene(vp39)of Leucania seperata nuclear polyhedrosisvirus has been leeated on PstI-F.BamHI-C,EcoRI-C,E,XhoI-D,I,EcoRV-H,X fragnients by Southern blot.Besides amplification of the predicted 1406bp fragment including intact major capsid proteingene of AcNPV using XhoI and HindⅢ-digested AcNPV DNA as a teniplate.a ca.400 bp fragmenthas been amplified.In this paper we disscused the PCR amplification of ca.400 bp fragment of bothspecificity and nonspocificity.

  • 加载中
  • 加载中

Article Metrics

Article views(2281) PDF downloads(759) Cited by()

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    PCR Amplification CloningProtein Gene and Location of Major Capsid Of Baculovirus

    • 1. Institute of Hology ,Wuhan University ,Wuhan 430072

    Abstract: he major eapeid protein gene(vp39)of Autographa californica nuclear polyhedrosis virus(AcNPV)was amplified successfully by PCR technique and cloned into pBluescript SK(+).With pure AcNPVvp39 gene as a probe,the major capeid protein gene(vp39)of Leucania seperata nuclear polyhedrosisvirus has been leeated on PstI-F.BamHI-C,EcoRI-C,E,XhoI-D,I,EcoRV-H,X fragnients by Southern blot.Besides amplification of the predicted 1406bp fragment including intact major capsid proteingene of AcNPV using XhoI and HindⅢ-digested AcNPV DNA as a teniplate.a ca.400 bp fragmenthas been amplified.In this paper we disscused the PCR amplification of ca.400 bp fragment of bothspecificity and nonspocificity.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return