PCR Amplification CloningProtein Gene and Location of Major Capsid Of Baculovirus

he major eapeid protein gene(vp39)of Autographa californica nuclear polyhedrosis virus(AcNPV)was amplified successfully by PCR technique and cloned into pBluescript SK(+).With pure AcNPVvp39 gene as a probe,the major capeid protein gene(vp39)of Leucania seperata nuclear polyhedrosisvirus has been leeated on PstI-F.BamHI-C,EcoRI-C,E,XhoI-D,I,EcoRV-H,X fragnients by Southern blot.Besides amplification of the predicted 1406bp fragment including intact major capsid proteingene of AcNPV using XhoI and HindⅢ-digested AcNPV DNA as a teniplate.a ca.400 bp fragmenthas been amplified.In this paper we disscused the PCR amplification of ca.400 bp fragment of bothspecificity and nonspocificity.