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Volume 09 Issue 2
April 1994
    Citation: CHENG Ning-Hui, PU Jie-Qin, FANG Zhong-Da. Studies on the TN Strain of Cucumber Mosaic Virus Inducing Tomato Necrosis Disease .VIROLOGICA SINICA, 1994, 9(2) : 143-150.
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    Studies on the TN Strain of Cucumber Mosaic Virus Inducing Tomato Necrosis Disease

    • 1.

      Department of plant proteation ,Nanjing Agricuttural University ,Nanjing 210095

    • Available online: 05 June 1994
    • lethal disease of tomato eecurred severely in the vicinity of Nanjing from 1991 to 1992 wascaused by a viral pathogen.A virus was isolated from the diseased tomato collected from field and.called the isolate TN.It infected 8 families 26 species host plants out of 10 families 39 species inoculated in the host ranse test and induced necrosis in tomato, pepper and tobacco.Its stability in extractedsap was TIP 50℃,DEP 510-3-10-4,and LIV 24-48hrs.The virus transmitted by aphid in non-persistence and reacted positively with CMV antiserum in agar diffusion test.Purified virions of the isolate TN were obtained by differential centrifugation and its morphology was spherical about 28nm in diameter by electron microscopy.Analysis of SDS-PAGE revealed that virion capsid protein had a singlecomponent (monomer),which molecular weight was 27500D.Viral RNAs contained normal CMVRNA 1-4 and additional lower molecular weight RNA5 by polyacrylamide gel electrophoresis. Theseresults were concurrent with the previous reports that
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      Studies on the TN Strain of Cucumber Mosaic Virus Inducing Tomato Necrosis Disease

      • 1. Department of plant proteation ,Nanjing Agricuttural University ,Nanjing 210095

      Abstract: lethal disease of tomato eecurred severely in the vicinity of Nanjing from 1991 to 1992 wascaused by a viral pathogen.A virus was isolated from the diseased tomato collected from field and.called the isolate TN.It infected 8 families 26 species host plants out of 10 families 39 species inoculated in the host ranse test and induced necrosis in tomato, pepper and tobacco.Its stability in extractedsap was TIP 50℃,DEP 510-3-10-4,and LIV 24-48hrs.The virus transmitted by aphid in non-persistence and reacted positively with CMV antiserum in agar diffusion test.Purified virions of the isolate TN were obtained by differential centrifugation and its morphology was spherical about 28nm in diameter by electron microscopy.Analysis of SDS-PAGE revealed that virion capsid protein had a singlecomponent (monomer),which molecular weight was 27500D.Viral RNAs contained normal CMVRNA 1-4 and additional lower molecular weight RNA5 by polyacrylamide gel electrophoresis. Theseresults were concurrent with the previous reports that

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