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Volume 09 Issue 3
June 1994
    Citation: CHEN Wan-Rong, TIAN Xin, BAO Zuo-Xi, LI Jing-Yun, DENG Da-Rong. Partial genome sequence analysis of hepatitis E .virus isolated by cell culture .VIROLOGICA SINICA, 1994, 9(3) : 213.
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    Partial genome sequence analysis of hepatitis E .virus isolated by cell culture

    • 1.

    • Available online: 05 September 1994
    • Genome of presumed hepatitis E virus XJ90 and R25 was partially amplified by antigen capturepolymerase chain reaction(AC/PCR).cDNA band same as ET1·1 clune was ubserved.After purification,this cDNA band was sequenced using dideoxy mediated chain termination method.The nu-cleotide and amino acid homology between XJ90 R25 and ET1·1 clone were 99.6%,100%and99%,99%,respectively. This result showed that both XJ90 and R25 are hepatitis E virus.
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      Partial genome sequence analysis of hepatitis E .virus isolated by cell culture

      • 1. 

      Abstract: Genome of presumed hepatitis E virus XJ90 and R25 was partially amplified by antigen capturepolymerase chain reaction(AC/PCR).cDNA band same as ET1·1 clune was ubserved.After purification,this cDNA band was sequenced using dideoxy mediated chain termination method.The nu-cleotide and amino acid homology between XJ90 R25 and ET1·1 clone were 99.6%,100%and99%,99%,respectively. This result showed that both XJ90 and R25 are hepatitis E virus.

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