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Volume 09 Issue 3
June 1994
    Citation: CHEN Dong, XIE Tian-En, JIN Feng. Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene .VIROLOGICA SINICA, 1994, 9(3) : 217.
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    Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene

    • 1.

      Wuhan Institute of Virology,CAS,Wuhan 430071

    • Available online: 05 September 1994
    • Agrotis segetum granulosis virus (AsGV) is an important biolosical control agent for A.segelum.Several PstI fragments from AsGV were cloned into pUC19.Under the very low strinsency(20% formamide,37℃ ),we have leealized the granulin sene on BglII-S or T,EcoRI-A,PstI-A or B frag.ments of AsGV DNA,by using the 32P-labelled polyhedrin gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV)as a prcbe.
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      Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene

      • 1. Wuhan Institute of Virology,CAS,Wuhan 430071

      Abstract: Agrotis segetum granulosis virus (AsGV) is an important biolosical control agent for A.segelum.Several PstI fragments from AsGV were cloned into pUC19.Under the very low strinsency(20% formamide,37℃ ),we have leealized the granulin sene on BglII-S or T,EcoRI-A,PstI-A or B frag.ments of AsGV DNA,by using the 32P-labelled polyhedrin gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV)as a prcbe.

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