Citation: ZHOU Chang-Yong, DIAO Xua-Yuan, JIANG Yuan-Hui. Annual Change of ELISA Valu~ of Satsuma Dwarf Virus in Different Parts of Satsuma Budllng and of Citrus Tristeza Virus in Different Parts of Sweet Orange Seedling .VIROLOGICA SINICA, 1994, 9(3) : 239.

Annual Change of ELISA Valu~ of Satsuma Dwarf Virus in Different Parts of Satsuma Budllng and of Citrus Tristeza Virus in Different Parts of Sweet Orange Seedling

  • Available online: 05 September 1994
  • From September 1991 to August 1992,leaves,stem barks and roots collected in each monthfrom four-year-old satsuma budling infected with satsuma dwarf virus(SDV) and from four-year-oldsweet orange seedling infected with citrus tristza virus(CTV)were used for detecting SDV and CTVby using DAS-ELISA.The results show that SDV could not be detected in old leaves and stem barksall over the year.It could be stably detected in young leaves in March,April,May,June,Septemberand October but not in July and August,and in young steni barks it could be detected in April,May,June,July and Octuber,the concentration of SDV in young stem bark decreased sharply in July,Lowconcentratiun of SDV in roots could be detected in July and August as the new roots grow,This indicates that SDV propagated in young tissues under the terms of cool temperature.CTV could be stablydetedted not only in stem barks but also in leaves and roots all over the year,but the ELISA values(A410nm)decreased in summer and winter season,especially in old lea

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    Annual Change of ELISA Valu~ of Satsuma Dwarf Virus in Different Parts of Satsuma Budllng and of Citrus Tristeza Virus in Different Parts of Sweet Orange Seedling

    • 1. 

    Abstract: From September 1991 to August 1992,leaves,stem barks and roots collected in each monthfrom four-year-old satsuma budling infected with satsuma dwarf virus(SDV) and from four-year-oldsweet orange seedling infected with citrus tristza virus(CTV)were used for detecting SDV and CTVby using DAS-ELISA.The results show that SDV could not be detected in old leaves and stem barksall over the year.It could be stably detected in young leaves in March,April,May,June,Septemberand October but not in July and August,and in young steni barks it could be detected in April,May,June,July and Octuber,the concentration of SDV in young stem bark decreased sharply in July,Lowconcentratiun of SDV in roots could be detected in July and August as the new roots grow,This indicates that SDV propagated in young tissues under the terms of cool temperature.CTV could be stablydetedted not only in stem barks but also in leaves and roots all over the year,but the ELISA values(A410nm)decreased in summer and winter season,especially in old lea

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