Cytoplasmic Mol’pl10gen is of Infectious Canine HepatRis Virus in Dog Kidney Cells

The morphogenesis and antigen localization of infectious canine hepatitis virus(ICHV)in dog kidney cells were studied with electron microscopy and immunogold electron microscopy,Apart from theassembly of ICHV in nuclei,virions were also assembled in cytoplasm of the infected cells.The modeof viral morphogenesis in homogeneous dense inclusions and paracrystalline ones in cytoplasm was similar to that well known in nuclei.Result of immunogold labelling showedthathere there were a lot of anti-genic components of ICHV providing the viral structural protein for the assembly of nucleocapsids inthese two kinds of inclusions in cytoplasm,In addition,some virus core-like electron opaque structuresaround 50nm in diameter were presented in cytoplasm as those in nuclei.