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Volume 09 Issue 3
June 1994
    Citation: SHAO Xin-Wu, DAO Pei-Zhen, GUO Ju-Chao, CHEN Hong-Shan. Isolation，Purification and DNA Polymerase Activity Analysis of DI-IBV Repficatlve Complexe .VIROLOGICA SINICA, 1994, 9(3) : 275.
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    Isolation，Purification and DNA Polymerase Activity Analysis of DI-IBV Repficatlve Complexe

    • 1.

      Institute of Medical Biotechnology,Chinese Academy of Medical Sciences,Beijing 100050

    • Available online: 05 September 1994
    • Duck hepatitis B virus re plicativc complexe~ (DHBV RCs)wcl~e isol~ted from DI-~V—infected duck liver by chromato~aphy thxou Bio--Oel A 5m followed crose gradient centrifugation．The feIathe endogenous DNA polymcra．se activity of the purified RCs was 100 tim es higher than that of the unchromatosraphed one，confirmed by detecting their DHBcAg with Dot-EIA method．
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      Isolation，Purification and DNA Polymerase Activity Analysis of DI-IBV Repficatlve Complexe

      • 1. Institute of Medical Biotechnology,Chinese Academy of Medical Sciences,Beijing 100050

      Abstract: Duck hepatitis B virus re plicativc complexe~ (DHBV RCs)wcl~e isol~ted from DI-~V—infected duck liver by chromato~aphy thxou Bio--Oel A 5m followed crose gradient centrifugation．The feIathe endogenous DNA polymcra．se activity of the purified RCs was 100 tim es higher than that of the unchromatosraphed one，confirmed by detecting their DHBcAg with Dot-EIA method．

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