Citation: WANG Ming-Shu, GUO Wan-Zhu, LOU Gao-Ming, BI En-Ge, XUAN Hua. Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping .VIROLOGICA SINICA, 1994, 9(4) : 347.

Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping

  • Available online: 10 June 2015
  • The Restriction endonuclease fragments except the ones from BamH I-1,2 were inserted into plasmid pBR322.The genomic library of Pseudurabies virus Min-A was also constructed.The Cloned frag-ment degested by BamH I were ldbeled with pliotobiotin,With molecular hybridization,the maincleavage site of Pseudorabies virus Min-A strain were determined.

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    Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping

    • 1. Sichuan Agricultural University

    Abstract: The Restriction endonuclease fragments except the ones from BamH I-1,2 were inserted into plasmid pBR322.The genomic library of Pseudurabies virus Min-A was also constructed.The Cloned frag-ment degested by BamH I were ldbeled with pliotobiotin,With molecular hybridization,the maincleavage site of Pseudorabies virus Min-A strain were determined.

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