Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping
Abstract: The Restriction endonuclease fragments except the ones from BamH I-1,2 were inserted into plasmid pBR322.The genomic library of Pseudurabies virus Min-A was also constructed.The Cloned frag-ment degested by BamH I were ldbeled with pliotobiotin,With molecular hybridization,the maincleavage site of Pseudorabies virus Min-A strain were determined.