Gone-protein—coding Assignments of Grass Carp Hemorrhage Virus Genome RNA Species

ll 11 segments of GCHV(grass.carp hemorrhage virus)873 dsRNA were isolated and individual-ly retrieved by SDS-PAGE and electric elution.Each viral genome segment in 90%DMSO denaturedby heating at 45℃ for l5 min were translated in the wheat germ tramlation system at 25℃ for 70min.The translation products were analysed in above system and l2 protein bands were displayed onthe X-ray film,RNA segments 1,2,3,4,5 and 10 coded individually for structural proteins of the in-ner virus shell,namely VP1,VP2,VP3,VP4,VP5 and VP10.RNA species coding for outer shell protenisVP6 and VP7 which are structural proteins were assigned to segments 6 and 7.Segments 8 and 9 codedindividually for VP52kD and VP 4l kD,and segment ll coded for VP29kD and VP 19.5kD.But weare not sure wether VP52kD,VP41kD,VP29kD and VP19.5kD are structural proteins or not.