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Volume 10 Issue 2
April 1995
    Citation: MI Ke-Yong, WANG Qiang, ZHU Yong, REN Xiang-Yuan, YANG Yu-Ying. Construction of a cDNA Libray of the Bovine Viral Diarrhea Virus Genome .VIROLOGICA SINICA, 1995, 10(2) : 161.
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    Construction of a cDNA Libray of the Bovine Viral Diarrhea Virus Genome

    • 1.

      Wuhan Institute of Virology

    • 2.

      Academia Sinica

    • Available online: 05 June 1995
    • ovine viral diarrhea virus(BVDV)InL genomic RNA was used as a template to synthesizeDNA by reverse transcriptase.The ds cDNA was synthesized by RNase H-DNA polymerase,and waslsetrted into the Pst I site of the pUC8 vector by dC-dG homopolymeric tailing and ligation.After beig transformed to E.coli JM101,the recombinants were screened by in situ hybridization with γ-￣(32)P-ATP labeled purified BVDV RNA,Restrict ion enzyme ana lysis sho wed that the largest cDNA insertwas 12kb-13kb,it almust covered the BVDV...
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      Construction of a cDNA Libray of the Bovine Viral Diarrhea Virus Genome

      • 1. Wuhan Institute of Virology
      • 2. Academia Sinica

      Abstract: ovine viral diarrhea virus(BVDV)InL genomic RNA was used as a template to synthesizeDNA by reverse transcriptase.The ds cDNA was synthesized by RNase H-DNA polymerase,and waslsetrted into the Pst I site of the pUC8 vector by dC-dG homopolymeric tailing and ligation.After beig transformed to E.coli JM101,the recombinants were screened by in situ hybridization with γ-￣(32)P-ATP labeled purified BVDV RNA,Restrict ion enzyme ana lysis sho wed that the largest cDNA insertwas 12kb-13kb,it almust covered the BVDV...

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