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Volume 10 Issue 2
April 1995
    Citation: GONG Xiao-Meng, DU Nian-Xin, CAO Jia, CHEN Qing-Han, SONG De-Xiu. Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity .VIROLOGICA SINICA, 1995, 10(2) : 177.
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    Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity

    • 1.

      Department of Veterinary Microbiology and Immunology. Nanjing Agricultural.University

    • 2.

      Nanjing 210095Cao Jie

    • Available online: 05 June 1995
    • Poly(A)￣+RNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)￣+RNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with ...
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      Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity

      • 1. Department of Veterinary Microbiology and Immunology. Nanjing Agricultural.University
      • 2. Nanjing 210095Cao Jie

      Abstract: Poly(A)￣+RNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)￣+RNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with ...

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