Citation: HONG Shi-Wen, HE Hong-Xia, SHU Feng-Lan, LIN Tao. A Study on the Detection of Antibodies to HIV Type 1 and Type 2 by Using Synthetic Peptides .VIROLOGICA SINICA, 1995, 10(3) : 236.

A Study on the Detection of Antibodies to HIV Type 1 and Type 2 by Using Synthetic Peptides

  • Available online: 05 September 1995
  • Two peptides of human immunodeficiency virus(HIV)type 1 and type 2 were synthe-sized by solid-phase method. An indirect enzyme-linked immunosorbent assay(ELISA)em-ploying these peptides as an antigen adsorbent was developed to detect the infections of HIV-1 and HIV-2.A comparison of our reagent with UBI reagent in detection of 46 positive serumspecimens and 94 negative control serum specimens for HIV-1 and HIV-2 showed that theconcident rate for positive was 97.8%, for negative was 100%,and the general coincidentrate was 99.3%. The results indicated that this method can be used in detection for infectionsof HIV-1 and HIV-2.

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    A Study on the Detection of Antibodies to HIV Type 1 and Type 2 by Using Synthetic Peptides

    • 1. d hospital of PLA,Beijing 100039

    Abstract: Two peptides of human immunodeficiency virus(HIV)type 1 and type 2 were synthe-sized by solid-phase method. An indirect enzyme-linked immunosorbent assay(ELISA)em-ploying these peptides as an antigen adsorbent was developed to detect the infections of HIV-1 and HIV-2.A comparison of our reagent with UBI reagent in detection of 46 positive serumspecimens and 94 negative control serum specimens for HIV-1 and HIV-2 showed that theconcident rate for positive was 97.8%, for negative was 100%,and the general coincidentrate was 99.3%. The results indicated that this method can be used in detection for infectionsof HIV-1 and HIV-2.

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