Rapid Detection of Proviral DNA of Bovine Leukaemia Virus Using Nested PCR

This paper reported that proviral DNA of bovine leukaemia virus(BLV)was detected bynested PCR.External and internal primers were selected from gp51 gene in BLV genome,Theproducts of the nested PCR were identified by 2%a8arose 8el electrophoresis and were com-flrmed by hybridlzation with a biotinylated oligonucleotide probe,The results of the nested PCRshow that DNA forms of the provirus were detected from two host cells.