Citation: XIAO Lian-Chun, SHI Zheng-Li, GAO Wei, ZHANG Li-Ren, CHEN Dai-Hua. Isolation,Putjfication of Pem em chinesis Parvovirus and Analysis of its Nueleic Acid and Protein .VIROLOGICA SINICA, 1995, 10(4) : 356.

Isolation,Putjfication of Pem em chinesis Parvovirus and Analysis of its Nueleic Acid and Protein

  • Available online: 05 December 1995
  • Small spherical virus with diameter of ca,20 nm was isolated from infected shrimps.Thevirions were icosahedron and naked. The infection exDeriment showed that the mortality was upto 66%.The viral nucleic acid was ssDNA after digested with DNase,RNase and Sl nuclease,Analysis of viral polypeptides by SDS-PAGE revealed that the virion had four structural polypep-tides with MW 86 kD,79kD,70 kD and 25.5 kD.These resul ts were in comformity with Par-voviridae,thus it was proposed that the virus be named Penaeus chinesis Parvovirus.

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    Isolation,Putjfication of Pem em chinesis Parvovirus and Analysis of its Nueleic Acid and Protein

    • 1. Wuhan lnstitute of Virology,Academia Sinica,Wuhan 430071

    Abstract: Small spherical virus with diameter of ca,20 nm was isolated from infected shrimps.Thevirions were icosahedron and naked. The infection exDeriment showed that the mortality was upto 66%.The viral nucleic acid was ssDNA after digested with DNase,RNase and Sl nuclease,Analysis of viral polypeptides by SDS-PAGE revealed that the virion had four structural polypep-tides with MW 86 kD,79kD,70 kD and 25.5 kD.These resul ts were in comformity with Par-voviridae,thus it was proposed that the virus be named Penaeus chinesis Parvovirus.

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