Isolation,Putjfication of Pem em chinesis Parvovirus and Analysis of its Nueleic Acid and Protein
Abstract: Small spherical virus with diameter of ca,20 nm was isolated from infected shrimps.Thevirions were icosahedron and naked. The infection exDeriment showed that the mortality was upto 66%.The viral nucleic acid was ssDNA after digested with DNase,RNase and Sl nuclease,Analysis of viral polypeptides by SDS-PAGE revealed that the virion had four structural polypep-tides with MW 86 kD,79kD,70 kD and 25.5 kD.These resul ts were in comformity with Par-voviridae,thus it was proposed that the virus be named Penaeus chinesis Parvovirus.