Citation: WANG Chun-Jie, WANG Wen-Liang, WANG Bo-Yun. Detection of HCV RNA and its NS3 antigen in human primary intrahepato-cholangiocarcinoma tissues .VIROLOGICA SINICA, 1996, 11(1) : 29.

Detection of HCV RNA and its NS3 antigen in human primary intrahepato-cholangiocarcinoma tissues

  • Available online: 05 March 1996
  • CV RNA and its NS3 region C33c antigen were detected by in situ hybridization withdigoxgenin-11-dUTP labeled HCV 5'non coding region cDNA probe and immunohistochemicalmethods with monoclonal antibody against recombinant HCV C33c protein in 35 cases of primaryintrahepatcr-cholangiocarcinoma(PIC) tissues.The positive rate of HCV RNA in PIC was 83%(29/35) and the HCV RNA was localized in the cytoplasm and/or nucleus of cancerous cell andhepatocytes.In one case,HCV RNA was also detected in the nuclus of lymphocyte and sinusoidalcell/or kupffer′S cells.The positive rate of HCV NS3 region C33c antigen was 89%(31/35)inPIC,the positive reaction was found in the cytoplasm of cancerous cell and hepatocytes,the posi-tive cells distributed in local and diffusely.HBxAg was also detected in 77%(37/45)of the pa-tients studied.Our results suggest that HCV infection has closely relationship with the develop-ment of PIC except HBV infection.HCV infection may play an important role in the carcinogene-sis of PIC.

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    Detection of HCV RNA and its NS3 antigen in human primary intrahepato-cholangiocarcinoma tissues

    • 1. Department of Pathology.Fourth Military Medical University.Xi’an 710032

    Abstract: CV RNA and its NS3 region C33c antigen were detected by in situ hybridization withdigoxgenin-11-dUTP labeled HCV 5'non coding region cDNA probe and immunohistochemicalmethods with monoclonal antibody against recombinant HCV C33c protein in 35 cases of primaryintrahepatcr-cholangiocarcinoma(PIC) tissues.The positive rate of HCV RNA in PIC was 83%(29/35) and the HCV RNA was localized in the cytoplasm and/or nucleus of cancerous cell andhepatocytes.In one case,HCV RNA was also detected in the nuclus of lymphocyte and sinusoidalcell/or kupffer′S cells.The positive rate of HCV NS3 region C33c antigen was 89%(31/35)inPIC,the positive reaction was found in the cytoplasm of cancerous cell and hepatocytes,the posi-tive cells distributed in local and diffusely.HBxAg was also detected in 77%(37/45)of the pa-tients studied.Our results suggest that HCV infection has closely relationship with the develop-ment of PIC except HBV infection.HCV infection may play an important role in the carcinogene-sis of PIC.

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