The application of PCR-ELISA method for detecting hepatitis C virus in serum

Genome of the characters for the viral multiplication and cytopathic changes in Bs484 cells afterinfection with Buzura suppressaria nuclear polyhedrosis viruses(BsNPV)were reported in thispaper,The results were as follow:1.The multiplication of progeny viruses showed differentchangeable forms in the different times postinfection and the amounts of viral multiplication afterinfection with BsNPV were linearly relating to the viral infective concentration in certain range.2.The extracellular viruses in supernatant from culture were examined at 12 hours postinfection.3.The best fitted developing temperature of BsNPV in Bs484 cells was 24～26℃with regards tothe viral multiplication in vitro.4.The distinct charactistics of cytopathic changes after infectionwith BsNPV mainly included a amounts of polyhedra in nucleus,gathered infected cells and a in-flated cell with big nucleus.In addition,the two types of infected cells were observed in experi-men:a type of nucleus contained many polyhedra,another only had few polyhedr