Genomic Typing and Sequence Analysis of Hantavirus

The present study reports the use of reverse transcriptase polymerase chain reaction(RT-PCR)for typing 18 independently isolated Hantaviruses from Asia-Pacific area by 2 sets of type-specific primers and the sequence analysis.The results of PCR showed that Hantaan specificprimers could amplify all 10 isolates in the Hantaan serotype,while Seoul specific primers werereactive to all 7 isolates in Seoul serotype and Leakey.No cross-reaction was obserred in the ex-periment.The PCR products were analysed by nested PCR and restriction endonuclease digestionfor further confirmation。The sequence analysis of PCR products revealed that the sequence simi-larities of R36 between 76-118 and R22 were 78.4% and 68.1%,respectively. In addition,apairwise comparison of sequence homology revealed that R36 shares a higher degree of sequencehomology with isolates in Hantaan serotype than those in Seoul serotype.Lekey could be ampli-fied by Seoul specific primers