Study on ELISA method for detecting specific circulating immune complexes in sera of patients with HFRS

new ELISA method was established for detecting specific IgG,IgM,IgA type circulatingimmune complexes(CIC)in sera of patients with Hemorrhagic Fever with Renal Sydrome(HFRS).The rabbit anti-HFRS virus antibody was used as coated antibody and anti-humanIgG,IgM,IgA monoclonal antibodies were used as HRP conjugates.The results showed that thedetected rates of specific IgG,IgM,IgA types CIC in 73 acute phases(1￣8days after illness)were 69.49%,69.86% and 56.16% respectively,in 54 convalescence phases(10￣28 days afterillness)were 90.74%,85.19% and 75.93% respectively,28 sera in 1￣3 years after illness wereall 0.00%,36 serum specimens from normal and hepatitis B patients were all negative.The developed method showed highl specificity and sensitirity as well as reproducibility(CV:IGG12.07%,IgM6.29% and IgA12.89%)for the detection of HFRS specific CIC.