Virus isolation from the peripheral blood mononuclear cells of goats experimentally infected with ovine progressive pneumonia virus
Abstract: The viruses were isolated by cocultivation of sheep foetal lung cells and the peripheral blood mononuclear cells of goats inoculated with ovine progressive pneumonia virus (OPPV). The result indicates that OPPV can infect goats. The isolated viruses were characterized by observations of cytopathogenic effects, indirect fluorescent antibody assay, electron microscopy, and polymerase chain reaction (PCR), and were further identified as OPPV. The isolation results show that this is a sensitive method of isolating OPPV. Sheep foetal lung cells can be passaged for forty times, and each subculture can be used to isolate virus, so it is a very practical method of isolating OPPV.