Citation: LI Ru-Lin, WANG Hai-Tao, WAN Ze-Sheng, XU De-Zhong. Molecular cloning and sequencing of ribozyme region of HDV from a serious hepatitis patient .VIROLOGICA SINICA, 1997, 12(3) : 235.

Molecular cloning and sequencing of ribozyme region of HDV from a serious hepatitis patient

  • Available online: 05 September 1997
  • The cDNA (651nt - 941nt) fragment of an isolate of hepatitis delta virus with 289 bp was determined by cloning and Sequencing, from a HBsAg carrier positive both for anti - HDAg and HDV RNA from China, Sichuan Province, using reverse transcription polymerase chain reaction.It contains the region of HDV ribozyme and its self - cleavage site. Comparing this Sichuan isolate with other known HDV isolates from Henan, US - 1, Japan, Peru and Taiwan, the result showed that the homology of nucbotides is 97. 2 %, 93 %, 94 %, 79 %, 96 %, respectively.

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    Molecular cloning and sequencing of ribozyme region of HDV from a serious hepatitis patient

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    Abstract: The cDNA (651nt - 941nt) fragment of an isolate of hepatitis delta virus with 289 bp was determined by cloning and Sequencing, from a HBsAg carrier positive both for anti - HDAg and HDV RNA from China, Sichuan Province, using reverse transcription polymerase chain reaction.It contains the region of HDV ribozyme and its self - cleavage site. Comparing this Sichuan isolate with other known HDV isolates from Henan, US - 1, Japan, Peru and Taiwan, the result showed that the homology of nucbotides is 97. 2 %, 93 %, 94 %, 79 %, 96 %, respectively.

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