Citation: HAN Jian-Qiu, JackT.Stapleton. Assay of amino acid mutagenesis in hepatitis A virus cellular receptor binding region .VIROLOGICA SINICA, 1997, 12(3) : 239.

Assay of amino acid mutagenesis in hepatitis A virus cellular receptor binding region

  • Available online: 05 September 1997
  • Point mutation was produced in the cellular receptor binding region of hepatitis A virus VP1 based on PCR directed mutagenesis. The results showed that the replication kinetics were changed when 1143, 1187, 1202 and 1225 amino acid were mutated respectively. HAV replicatioll quantity decreased at a different range compared with prototype HAV strain. The results indicated that these amino acid may be involved in the binding of cellular receptor, The amino acid substitution may affect the attachment and the uncoating process of HAV to permissive cell,therefore the virus infection decreased.

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    Assay of amino acid mutagenesis in hepatitis A virus cellular receptor binding region

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    Abstract: Point mutation was produced in the cellular receptor binding region of hepatitis A virus VP1 based on PCR directed mutagenesis. The results showed that the replication kinetics were changed when 1143, 1187, 1202 and 1225 amino acid were mutated respectively. HAV replicatioll quantity decreased at a different range compared with prototype HAV strain. The results indicated that these amino acid may be involved in the binding of cellular receptor, The amino acid substitution may affect the attachment and the uncoating process of HAV to permissive cell,therefore the virus infection decreased.

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