Citation: YAN Xi-Tang, WANG Jin-Fang, QIU Bing-Sheng, TIAN Po. Resistance to rice stripe virus conferred by expression of coat protein in transgenic Indica rice plants regenerated from bombarded suspension culture .VIROLOGICA SINICA, 1997, 12(3) : 260.

Resistance to rice stripe virus conferred by expression of coat protein in transgenic Indica rice plants regenerated from bombarded suspension culture

  • Available online: 05 September 1997
  • The coat prolein (CP) gene of a Chinese isolate of rice stripe virus (RSV) was synthesized,cloned and sequenced. Embryogenic suspension cultures were initialed from calli which derived from Indiea rice mature embryo. For bombardment of cell suspension culture, tungsten particles of diameter of 1.09 μm were coated with DNA of the expression vector pRoK Ⅱ harbouring CP coding sequence downstream CaMV 35S promoter. Bombarded cultures were selected by growing in the medium containing G418 (40mg/mL).Ten plantlets regenerated from G418 resistant calli were analysed by Southem blot Date of receiption: 1996 -09-13, date of correction: 1997-02- 26 This work was supported by grant RF 95001 # 309 from the Rockfeller FoundationTo whom correspondence should be addressedlLsing 32P - dtryP 1abelled CP gene as probe. The genomic DNAs extrac1cd frotn resistant as well as th`>control plants were digested with EcoRI and BamHI. Samples from two plants showed tw0 hyl,ridlzat1onbands with the sizes 0f 0. 6 kb and 0. 7 kb correspo

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    Resistance to rice stripe virus conferred by expression of coat protein in transgenic Indica rice plants regenerated from bombarded suspension culture

    • 1. Inst. Microbiology, Chinese Academy of Sciences

    Abstract: The coat prolein (CP) gene of a Chinese isolate of rice stripe virus (RSV) was synthesized,cloned and sequenced. Embryogenic suspension cultures were initialed from calli which derived from Indiea rice mature embryo. For bombardment of cell suspension culture, tungsten particles of diameter of 1.09 μm were coated with DNA of the expression vector pRoK Ⅱ harbouring CP coding sequence downstream CaMV 35S promoter. Bombarded cultures were selected by growing in the medium containing G418 (40mg/mL).Ten plantlets regenerated from G418 resistant calli were analysed by Southem blot Date of receiption: 1996 -09-13, date of correction: 1997-02- 26 This work was supported by grant RF 95001 # 309 from the Rockfeller FoundationTo whom correspondence should be addressedlLsing 32P - dtryP 1abelled CP gene as probe. The genomic DNAs extrac1cd frotn resistant as well as th`>control plants were digested with EcoRI and BamHI. Samples from two plants showed tw0 hyl,ridlzat1onbands with the sizes 0f 0. 6 kb and 0. 7 kb correspo

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