Citation: YUE Li-Li, JI Xi-Peng, DENG Yan-Hui, LV Li-Qun, DENG Xiao-Zhao. Fusion of HBV e Gene with Green Fluorescent Protein Gene and Its Expression in E. coli Cells .VIROLOGICA SINICA, 1997, 12(4) : 336.

Fusion of HBV e Gene with Green Fluorescent Protein Gene and Its Expression in E. coli Cells

  • Available online: 05 December 1997
  • The bifunction fusion protein of hepatitis B virus e antigen and mutant green fluorescent protein was expressed in E. coli BL21 strain. The fusion protein was separated and its anti-genecity was determined by ELISA kit for HBe, meanwhile the intensity of its luminescence could be observed. The approach in which the antigen was tagged by equal molecular GFP allows using the bi-function fusion protein to establish a new method for immunogical diagnosis.

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    Fusion of HBV e Gene with Green Fluorescent Protein Gene and Its Expression in E. coli Cells

    • 1. Institute of Virology
    • 2. Wuhan University
    • 3. Wuhan 430072Nanjing Institute of Military Medical Sciences
    • 4. Nanjing 210002

    Abstract: The bifunction fusion protein of hepatitis B virus e antigen and mutant green fluorescent protein was expressed in E. coli BL21 strain. The fusion protein was separated and its anti-genecity was determined by ELISA kit for HBe, meanwhile the intensity of its luminescence could be observed. The approach in which the antigen was tagged by equal molecular GFP allows using the bi-function fusion protein to establish a new method for immunogical diagnosis.

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