Citation: Li-Jing-Yun, WANG Hong-Xia, BAO Zuo-Xi. The Establishment and Application of Immunofluorescence Assay for Detection of Antibodies to HIV-1 .VIROLOGICA SINICA, 1997, 12(4) : 371.

The Establishment and Application of Immunofluorescence Assay for Detection of Antibodies to HIV-1

  • Available online: 05 December 1997
  • Using the mixture of HIV -1 infected and no - infected HeLa CD4+ cells, the antigen slides were prepared and the immunofluorescence assay for detection of antibodies to human immunode-ficiency virus type 1 was established. Seventy - seven serum samples including the national stan-dard reference of HIV antibody reagents were tested. The sensitivity and specificity were 96. 4 %and 100%, respectively. Compared with the confirmation test of Western Blot, the coincident rate was 92. 6 %. The results indicated th...

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    The Establishment and Application of Immunofluorescence Assay for Detection of Antibodies to HIV-1

    • 1. Institute of Microbiology and Epidemiology

    Abstract: Using the mixture of HIV -1 infected and no - infected HeLa CD4+ cells, the antigen slides were prepared and the immunofluorescence assay for detection of antibodies to human immunode-ficiency virus type 1 was established. Seventy - seven serum samples including the national stan-dard reference of HIV antibody reagents were tested. The sensitivity and specificity were 96. 4 %and 100%, respectively. Compared with the confirmation test of Western Blot, the coincident rate was 92. 6 %. The results indicated th...

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