Serving the Society Since 1986
Advanced Search
Volume 13 Issue 1
February 1998
    Citation: LEI Wan-Li, TANG Jia-Qi, LU Cheng-Beng, PAN Xiu-Zhen, LI Xian-Fu. Detection and Genotyping of Hantaviruses Using Reverse Transcription·Polym erase Chain Reaction .VIROLOGICA SINICA, 1998, 13(1) : 28.
    shu

    Detection and Genotyping of Hantaviruses Using Reverse Transcription·Polym erase Chain Reaction

    • 1.

      East-China Medical Istitute of Biological Techniques，Nanjing 210002College of Veterinary Medicine，Nanjing Agrieultural University．Najing 210095

    • Available online: 05 March 1998
    • Common primers were designed, based on M segmental sequences of two HFRSV serotypes—HTNV and SEOV, and RT PCR (reverse transcription-polymerase chain reaction) method was applied in detecting 39 strains of isolated hosts from different areas. Thirty six of the 39 samples were tested by cELISA (capture ELISA) in duplicated wells, while P/N≥2.10 and P-N≥0.10 the result was considered to the positive. The detection rates of RT PCR and cELISA were 97.6% and 82.4% respectively, 84.6% in correspondence. Fifteen of 38 RT PCR products could be digested by restriction endonuclease AluI. According to the restriction map the HFRSV detected was classified into two types——HTNV and SEOV.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(4244) PDF downloads(883) Cited by()

    Related
    Proportional views

      Detection and Genotyping of Hantaviruses Using Reverse Transcription·Polym erase Chain Reaction

      • 1. East-China Medical Istitute of Biological Techniques，Nanjing 210002College of Veterinary Medicine，Nanjing Agrieultural University．Najing 210095

      Abstract: Common primers were designed, based on M segmental sequences of two HFRSV serotypes—HTNV and SEOV, and RT PCR (reverse transcription-polymerase chain reaction) method was applied in detecting 39 strains of isolated hosts from different areas. Thirty six of the 39 samples were tested by cELISA (capture ELISA) in duplicated wells, while P/N≥2.10 and P-N≥0.10 the result was considered to the positive. The detection rates of RT PCR and cELISA were 97.6% and 82.4% respectively, 84.6% in correspondence. Fifteen of 38 RT PCR products could be digested by restriction endonuclease AluI. According to the restriction map the HFRSV detected was classified into two types——HTNV and SEOV.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return