Citation: LEI Juan-Li, CHEN Jiong, CHEN Jian-Ping, ZHENG Tao, CHENG Ye. Identification of Chinese Fungal·transm itted Filamentous W heat M osaic Virus by RT·PCR and SSCP .VIROLOGICA SINICA, 1998, 13(1) : 96.

Identification of Chinese Fungal·transm itted Filamentous W heat M osaic Virus by RT·PCR and SSCP

  • Available online: 05 March 1998
  • There are two filamentous wheat mosaic viruses transimitted by the plasmodiophoraceous fungus Polymyxa graminis :one is wheat spindle streak mosaic virus (WSSMV)first reported in Canada and the other is wheat yellow mosaic virus (WYMV) first reported in Japan .These viruses are very similar in particle morphology,biological and serological properties, but different in genomic sequences. The results of RT-PCR and single strand conformation polymorphism analysis (SSCP) indicated that the fungal transmitted filamentous wheat mosaic viral pathogen occurred in successive crops at different sites in China is wheat yellow mosaic virus. SSCP analysis with the fragments amplified using several primer sets all gave different patterns ,indicating the presence of sequence variations among eight isolates collected at different sites in China.

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    Identification of Chinese Fungal·transm itted Filamentous W heat M osaic Virus by RT·PCR and SSCP

    • 1. virology Laboratory ,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021

    Abstract: There are two filamentous wheat mosaic viruses transimitted by the plasmodiophoraceous fungus Polymyxa graminis :one is wheat spindle streak mosaic virus (WSSMV)first reported in Canada and the other is wheat yellow mosaic virus (WYMV) first reported in Japan .These viruses are very similar in particle morphology,biological and serological properties, but different in genomic sequences. The results of RT-PCR and single strand conformation polymorphism analysis (SSCP) indicated that the fungal transmitted filamentous wheat mosaic viral pathogen occurred in successive crops at different sites in China is wheat yellow mosaic virus. SSCP analysis with the fragments amplified using several primer sets all gave different patterns ,indicating the presence of sequence variations among eight isolates collected at different sites in China.

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