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Volume 13 Issue 4
August 1998
    Citation: LUAN Yi, YU Xiu-Ping, ZHAO Wei-Meng, GU Ji-Hui, ZHOU Ya-Bin. Human papillomavirus type 16 E6 gene's T-A cloning and expression in insect cell .VIROLOGICA SINICA, 1998, 13(4) : 305.
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    Human papillomavirus type 16 E6 gene's T-A cloning and expression in insect cell

    • 1.

      Department of Microbiology,Shandong Medical University

    • Available online: 05 December 1998
    • The baculovirus insect cell expression system was used to prepare the HPV16 E 6 recombinant protein. The HPV16 E 6 complete ORF amplified by PCR from HPV16 genome was T A cloned into the baculovirus transfer vector pVL1393 T under AcMNPV Polh. This recombinant transfer plasmid pVL1393 E 6 DNA and baculovirus DNA were co transfected into insect cell Sf 9. After the plaque screening, the recombinant baculovirus AcMNPV E 6 was expressed in insect cells. The E 6, analysised by SDS PAGE, was 18kD, and was recognized by antibody against E 6 protein, which showed their natural antigenecity.
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      Human papillomavirus type 16 E6 gene's T-A cloning and expression in insect cell

      • 1. Department of Microbiology,Shandong Medical University

      Abstract: The baculovirus insect cell expression system was used to prepare the HPV16 E 6 recombinant protein. The HPV16 E 6 complete ORF amplified by PCR from HPV16 genome was T A cloned into the baculovirus transfer vector pVL1393 T under AcMNPV Polh. This recombinant transfer plasmid pVL1393 E 6 DNA and baculovirus DNA were co transfected into insect cell Sf 9. After the plaque screening, the recombinant baculovirus AcMNPV E 6 was expressed in insect cells. The E 6, analysised by SDS PAGE, was 18kD, and was recognized by antibody against E 6 protein, which showed their natural antigenecity.

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