Anti-human immunodeficency virus activities of proteins from 17 species of plants
Abstract: In order to find new anti human immunodeficiency virus (HIV) ribosome inactivating proteins (RIPs), the anti HIV activities of 47 RIPs and crude proteins from 17 species of plants were examined by the following measurements: 1) the inhibition of syncytia formation; 2) the protection of HIV 1 infected cell;3) inhibition of HIV 1 infected cell fusion in coculture;4) reduction of p24 antigen expression and numbers of HIV antigen positive cells in acutely and chronically infected culture. Among 47 proteins, trichosanthin (TCS) (SI=193.3) from Trichosanthes kirilowii, protein fractions Ⅴ (SI=243) and Ⅵ (SI>1200) from Trichosanthes damiaoshanesis were found to obviously inhibit syncytia formation induced by HIV 1. Crotin Ⅰ from Croton tiglium, luffin from Luffa cylinarica , RIPs from odgsonia macrocarpa and Entada phaseoloides showed slightly inhibition of the syncytia formation (SI<50). Crude protein extracts from Trichosanthes Ovigera, Momordica macrophylla and an unnamed plant growing in