Cloning and expression of E7 gene of human papillomavirus type 16 (HB strain

HPV 16 (HB strain) E 7 gene was cloned into expression vector pWR590 1 by gene cloning technique. Recombinant plasmid pWHB E 7 was obstained by analysis of restrictional endonuclease. 70 kD HPV 16 (HB strain) E 7 fusion protein was expressed by E.coli JM 109 transfected with pWHB E 7. The recombinant protein could be recognized by standard E 7 monocloned antibody in Western blot. The production of E 7 recombinant protein was more than 30 percent of total E.coli protein by induction of IPTG. The E 7 fusion protein existing in inclusion body in E. coli could be conveniently extracted and purified.