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Volume 14 Issue 2
April 1999
    Citation: ZHANG Xiao-Dong, ZHANG Jia-Min, GUO Hai-Chao, SHU Li-Hua, HU Yuan-Yang. Rescue of Infectious Virions from Recom binant Plasmid with the Genome of Periplanta liginosa,Densovirus .VIROLOGICA SINICA, 1999, 14(2) : 152-156.
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    Rescue of Infectious Virions from Recom binant Plasmid with the Genome of Periplanta liginosa,Densovirus

    • 1.

      1nsitute Virology，Wuhan University，Wuhan 430072

    • Available online: 05 June 1999
    • An infectious genome of the Periplanta fuliginosa densovirus (PfDNV) has been cloned into the bacterial plasmid pUC119. The viral genome could be rescued from the recombinant plasmid PfNDV pUC119 by transfection to sensitive Periplanta fuliginosa larvae. Virions can be found in the cells of the transfected larvaes by electron microscope, and the rescued virions were as infectious as wild type virions. In contrast, the viral genome could not be rescued from the recombinant plasmid deleted one extremity.
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      Rescue of Infectious Virions from Recom binant Plasmid with the Genome of Periplanta liginosa,Densovirus

      • 1. 1nsitute Virology，Wuhan University，Wuhan 430072

      Abstract: An infectious genome of the Periplanta fuliginosa densovirus (PfDNV) has been cloned into the bacterial plasmid pUC119. The viral genome could be rescued from the recombinant plasmid PfNDV pUC119 by transfection to sensitive Periplanta fuliginosa larvae. Virions can be found in the cells of the transfected larvaes by electron microscope, and the rescued virions were as infectious as wild type virions. In contrast, the viral genome could not be rescued from the recombinant plasmid deleted one extremity.

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