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Volume 14 Issue 2
April 1999
    Citation: . Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells .VIROLOGICA SINICA, 1999, 14(2) : 181-184.
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    Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells

    • 1.

      Tangdu Hospital，The Fourth Military Medical University，Xi'an 710038

    • Available online: 05 June 1999
    • The coding region of S genorae segment of Hantaan virus(76／118 strain)was inserted into the eukarytic expression plasmld pVR1012 The recombinant expression plasmid pVRS22 was c。nstructed．Ver0一E cells were transiently tmnsfected in vitro with pVRS22 plasmid．The transient expresslon of Hantaan virus nucleocapsid proteins in Vero-E6 cells was detected by indi rect immunofluorescence asay(IFA)with monoclonal antibody 5H5 against Hantaan virus
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      Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells

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      • 1. Tangdu Hospital，The Fourth Military Medical University，Xi'an 710038

      Abstract: The coding region of S genorae segment of Hantaan virus(76／118 strain)was inserted into the eukarytic expression plasmld pVR1012 The recombinant expression plasmid pVRS22 was c。nstructed．Ver0一E cells were transiently tmnsfected in vitro with pVRS22 plasmid．The transient expresslon of Hantaan virus nucleocapsid proteins in Vero-E6 cells was detected by indi rect immunofluorescence asay(IFA)with monoclonal antibody 5H5 against Hantaan virus

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