Citation: JI Wei, WANG Qi-Huan, SHI Chong-Wen, XU Min, ZHANG Guo-Qiang, LIU Dan. Inhibition of Hepatitis B Virus by Retroviral Vectors Expressing Antisenses PreS/S .VIROLOGICA SINICA, 1999, 14(4) : 310-313.

Inhibition of Hepatitis B Virus by Retroviral Vectors Expressing Antisenses PreS/S

  • Available online: 05 December 1999
  • Retroviral vector expressing antisense RNA complementary to HBV preS/S (antisenseS) was used to transduce human hepatoblastoma cell 2. 2. 15, which was trans fected with HBVDNA and can express HBV markers. The results showed that the inhibitory effects of antisensegene transfer mediated by retroviral vector antisense-S on the expression of HBV antigens appeared as early as on day 3 after transduction, reached peak level on day 5, and persisted at leastfor 11 days. The inhibitory rates of HBsAg and HBeAg in the 2. 2. 15 cells by antisense-S were71% and 27% on day 5 after transduction, and in comparison to blank or sense-S, the inhibitoryeffects of antisense-S were highly significant (P0.01 ). The viability of transduced 2. 2. 15 cellswas not affected as indicated by MTT assays. The results demonstrated that retroviral vectorscontaining antisense gene can inhibit HBV, and the antisense retroviral vectors may be potentiallyuseful for anti-HBV gene therapy.

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    Inhibition of Hepatitis B Virus by Retroviral Vectors Expressing Antisenses PreS/S

    • 1. The Seventh Department ofInfectious Diseases,302 Hospital of the PLA,Beijing 100039
    • 2. Department of Infectious Diseases,The First Affiliated Hospital,Bdjing Medical University,Beijing 100034

    Abstract: Retroviral vector expressing antisense RNA complementary to HBV preS/S (antisenseS) was used to transduce human hepatoblastoma cell 2. 2. 15, which was trans fected with HBVDNA and can express HBV markers. The results showed that the inhibitory effects of antisensegene transfer mediated by retroviral vector antisense-S on the expression of HBV antigens appeared as early as on day 3 after transduction, reached peak level on day 5, and persisted at leastfor 11 days. The inhibitory rates of HBsAg and HBeAg in the 2. 2. 15 cells by antisense-S were71% and 27% on day 5 after transduction, and in comparison to blank or sense-S, the inhibitoryeffects of antisense-S were highly significant (P0.01 ). The viability of transduced 2. 2. 15 cellswas not affected as indicated by MTT assays. The results demonstrated that retroviral vectorscontaining antisense gene can inhibit HBV, and the antisense retroviral vectors may be potentiallyuseful for anti-HBV gene therapy.

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