Citation: LIN Han-Xin, LIN Ai-Tian, TUN Jie-Jian, LIN Ai-Yang, XIE Lian-Hui. Characterization of Proteins and Nucleic Acid of Rice Stripe Virus .VIROLOGICA SINICA, 1999, 14(4) : 343-352.

Characterization of Proteins and Nucleic Acid of Rice Stripe Virus

  • Available online: 05 December 1999
  • An isolate of rice stripe virus (designated as RSVYL) was purified. The particlesshowed to be pleomorphisms under electron microscope, mainly branched filamnts of about 80-250 nm in length and abeut 8 nm in width. There are also some open circular filaments of 3nm and 8 nm in width, and some filaments of 13 nm in width and 130-190 nm in length.The basic morphism of RSV particles should be filaments of 3 nm in width and various length.By SDS-PAGE analysis, the molecular weight of disease-specific protein (SP) encoded by vRNA4 was 19. 9 kDa and tha of coat protein (CP) encoded by vcRNA3 was 33. 6 kDa. Whennucleic acid extrcted from the purified RSV was electrophoresed under nondenaturing condition, the she of four dsRNAs(designated as dsRNA1-4 in order of decreasing size) was 4. 9106, 2. 7106, 2.0106 and 1. 7106 Da, respectively, and that of four ssRNAs (designated as ssRNA1-4 in order of decreasing size) was 3. 0106, 1. 2106, 0. 9 106 and 0. 8106 De, respectively. A fifth Segment with a size of 0. 58106

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    Characterization of Proteins and Nucleic Acid of Rice Stripe Virus

    • 1. Institute of Plant Virology of Fujian Agricultural University,Key Laboratory of Plant Virology of Fujian Province,Fuzhou 350002

    Abstract: An isolate of rice stripe virus (designated as RSVYL) was purified. The particlesshowed to be pleomorphisms under electron microscope, mainly branched filamnts of about 80-250 nm in length and abeut 8 nm in width. There are also some open circular filaments of 3nm and 8 nm in width, and some filaments of 13 nm in width and 130-190 nm in length.The basic morphism of RSV particles should be filaments of 3 nm in width and various length.By SDS-PAGE analysis, the molecular weight of disease-specific protein (SP) encoded by vRNA4 was 19. 9 kDa and tha of coat protein (CP) encoded by vcRNA3 was 33. 6 kDa. Whennucleic acid extrcted from the purified RSV was electrophoresed under nondenaturing condition, the she of four dsRNAs(designated as dsRNA1-4 in order of decreasing size) was 4. 9106, 2. 7106, 2.0106 and 1. 7106 Da, respectively, and that of four ssRNAs (designated as ssRNA1-4 in order of decreasing size) was 3. 0106, 1. 2106, 0. 9 106 and 0. 8106 De, respectively. A fifth Segment with a size of 0. 58106

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