Establishment of a ModeI for Proliferating White Spot Virusfor Syndrome virusln vivo

With white spot syndrome virus (WSSV) isolated from disease shrimp Penaeus chinensis, some of freashwater prawn and crabs: crayfish Cambarus clarkii, Macrobrachium rosenbergii, Macrobrachium nipponensis,Eriochier sinensis, Sinopotamon yangtsekiense were selected to be artificially infected. The result shows that only Cambarus clarkii can be infected byWSSV. The average mortality of three different dose groups (Group 1, 2, 3) was 94 % during 12days after injection. Intact viral particles can be seen from the hemolymph of dead or diseasedcrayfish using TEM negative staining, the shape of virion and their morphogenesis in host cell arequite similar or identical to the WSSV isolate. Hybridization in situ technique was used to confirm the reliability of the experiment. The result can be repeated using diseased Cambarus clarkiito infect the same kind of crayfish, it suggests crayfish Cambarus clarkii can be used as a goodmodel for the further study of WSSV.