Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou

The p74 gene of SpltNPV was located at EcoRI 4.4 kb,XhoI 4.9 kb and BamHI 3.0 kb fragments by Southern blotting with AcMNPV derived probe. After cloning of the three fragments, restriction enzyme analysis was used to construct their restriction map. A region of 2?545 bp including p74 gene of SpltNPV was sequenced. The open reading frame of SpltNPV p74 gene was decided to be 1?974 bp long, which codes 658 amino acids. Comparison of the homology with other baculovirus p74 genes shows that SpltNPV p74 is 64% and 63% identical to that of AcMNPV and CfMNPV at the amino acid level.