Cloning and sequencing of the Buzura suppressaria nucleopolyhedrovirus DNA polymerase gene

The DNA polymerase gene of Buzura suppressaria nucleopolyhedrovirus (BusuNPV) was amplified by PCR using virus DNA template. The amplified fragment was cloned into E.coli via plasmid pGEM T. The fragment of insert was analyzed on an automated DNA sequencer. Its sequence showed that the insert has 2 379 nucleotides in length and 793 aa was predicated. Comparing with other six members of Baculoviridae , amaino acid sequence of the DNA polymerase of BusuNPV has 57% similarity with HzSNPV, and 39.6% with OpMNPV.