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Volume 15 Issue 3
June 2000
    Citation: FANG Qi, AI Tao-Shan, JU Gui-Beng, YU Yun-Zhen, LIU Jia-Xin, HONG E-Beng. Study of the Siniperca chuatsi Viral Pathogen and its Infectious Characteristics in Fish Cells .VIROLOGICA SINICA, 2000, 15(3) : 297-301.
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    Study of the Siniperca chuatsi Viral Pathogen and its Infectious Characteristics in Fish Cells

    • 1.

      State Key Laboratory of Freshwater Ecologyand Biotechno[ogy，Instituteof Hydrobiology，Chinese Academy of Sciences， Wuhan 430072,China

    • Available online: 05 September 2000
    • An icosahedral virus was found in the ultra thin section of spleen, kidney, liver, gill of the outbreak infectious Siniperca chuatsi by electron microscope. The virions of section show hexagon, about 125 150 nm in diameter, and have envelope outside. Cell culture indicated that CIK cell was sensitive to the virus, which was isolated from the internal organs of Siniperca chuatsi. The CPE can be seen after being infected for 36 48 hours at 28℃. Cell cultured virus suspension is infectious and can be subcultured steadily in CIK cell line. Many virions isolated by different centrifugation from infected cell culture can be observed by negative stained electron microscope method, but no virions were detected in the control samples.
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      Study of the Siniperca chuatsi Viral Pathogen and its Infectious Characteristics in Fish Cells

      • 1. State Key Laboratory of Freshwater Ecologyand Biotechno[ogy，Instituteof Hydrobiology，Chinese Academy of Sciences， Wuhan 430072,China

      Abstract: An icosahedral virus was found in the ultra thin section of spleen, kidney, liver, gill of the outbreak infectious Siniperca chuatsi by electron microscope. The virions of section show hexagon, about 125 150 nm in diameter, and have envelope outside. Cell culture indicated that CIK cell was sensitive to the virus, which was isolated from the internal organs of Siniperca chuatsi. The CPE can be seen after being infected for 36 48 hours at 28℃. Cell cultured virus suspension is infectious and can be subcultured steadily in CIK cell line. Many virions isolated by different centrifugation from infected cell culture can be observed by negative stained electron microscope method, but no virions were detected in the control samples.

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