Citation: LAN Shui-Yun, ZHENG Ling-Ji, HU Yun-Wen, CHEN Chong, QIU Hai-Jun, YUAN Zheng-Hong. Expression of Hepatitis C Virus RNA Polym erase in Insect Cells .VIROLOGICA SINICA, 2000, 15(4) : 313-307.

Expression of Hepatitis C Virus RNA Polym erase in Insect Cells

  • Available online: 05 December 2000
  • The genes of C terminal of HCV NS3 region of two chronic patients in two different time were sequenced to analyse the conservation of a helper T cell epitope in HCV NS3 protein (amino acids 1248 to 1261). The epitope was synthesized and the immune response to it in a self limited patient and two chronic patients was detected using T lymphocyte proliferation assay and inhibition experiment. The epitope specific T cell line was established by several cycles of stimulation relax stimulation and analysed with a FACScan. The results indicated that the epitope didn't change in two chronic patients, all three patients had strong CD4 + T cell reponse to the epitope, and epitope specific CD4 + T cell line was established. These data suggest that the eptiope is a conserved strong helper T cell epitope and could become a promising candidate for designing a CD4 + T cell vaccine.

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    Expression of Hepatitis C Virus RNA Polym erase in Insect Cells

    • 1. Laboratory Medical Molecular Virology,Shah ai Medical Uniwersity,Shanghai.200032,China

    Abstract: The genes of C terminal of HCV NS3 region of two chronic patients in two different time were sequenced to analyse the conservation of a helper T cell epitope in HCV NS3 protein (amino acids 1248 to 1261). The epitope was synthesized and the immune response to it in a self limited patient and two chronic patients was detected using T lymphocyte proliferation assay and inhibition experiment. The epitope specific T cell line was established by several cycles of stimulation relax stimulation and analysed with a FACScan. The results indicated that the epitope didn't change in two chronic patients, all three patients had strong CD4 + T cell reponse to the epitope, and epitope specific CD4 + T cell line was established. These data suggest that the eptiope is a conserved strong helper T cell epitope and could become a promising candidate for designing a CD4 + T cell vaccine.

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