The Long-term Culture of hum an bone marrow stromai cells in vitro and their susceptibiiltty to viruses

To culture human bone marrow stromal cells long term in vitro , and investigate their susceptibility to virus, the fetus, child and adult bone marrow stromal cell were cultured for long time in vitro by the adherent assay. The myoid bone marrow stromal cells were obtained after being generated more than five times, then their susceptibility to five kinds of virus was assayed by microdose CPE. All myoid stromal cells were susceptible to VSV, PolioV, HSV I and Ⅱ. Except CoxB 3, producing obvious CPE with TCID 50 up to 10 -3 10 -4 . Fetal myoid stromal cells were two times higher than child, and four times than adult in terms of virus susceptibility. Our research supplies not only a simple. practicable assay for ex vivo long term culture of human bone marrow stromal cell, but also experimental reference for estimating the virus susceptibility of these cells, at the same time, contributes to a more extensive prospect for applying these cells.